An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

Authors

  • B. Tabanifar Dept. of Microbiology, School of Biology, Alzahra University, Tehran, Iran.
  • E. Asgarani Dept. of Genetics, School of Biology, Alzahra University, Tehran, Iran.
  • M. Faghihi Dept. of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • R, Salehi Dept. of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • T. Allame Dept. of Gynecology and Obstetrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract:

  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation. Results: This method produced DNA suitable for amplification using primers specific for human SMN and β globin genes in 98.63% and 82.2% of samples respectively. We also detected human papillomavirus DNA in 58.33% of appropriate samples. Conclusion: This procedure provides simple and efficient method for recovery of amplifiable genomic and viral DNA from paraffin wax embedded tissues.  

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Journal title

volume 3  issue 4

pages  173- 178

publication date 2008-09-01

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